Synthesis and Properties of Purine Nucleoside and Nucleotide Derivatives; New Nucleoside Analogues from Shikimic Acid 嘌呤核苷(酸)衍生物的合成及其性质研究莽草酸衍生的新型核苷类似物的合成研究
The purine and pyrimidine may be complexed with a sugar to produce a nucleoside. 嘌呤和嘧啶可以同一个糖复合,产生一个核苷。
Purine nucleoside phosphorylase inhibitor screening using immobilized enzyme reactor by capillary electrophoresis 基于毛细管电泳在柱酶微反应器技术筛选嘌呤核苷磷酸化酶抑制剂
Prokaryotic Expression and Functional Analysis of Purine Nucleoside Phosphorylase of the Key Enzyme for Ribavirin Produced 合成利巴韦林的关键酶嘌呤核苷磷酸化酶的原核表达及活性分析
Effect of gamma irradiation on erythrocyte purine nucleoside phosphorylase activity and erythrocyte membrane lipid peroxidation in vitro and in vivo 离体与整体照射对大鼠红细胞嘌呤核苷磷酸化酶活力与红细胞膜脂质过氧化值的影响
Purine nucleoside phosphorylase deficiency ( PNPdef) presenting with lymphopenia and developmental delay: Successful correction with umbilical cord blood transplantation 以淋巴细胞减少症和发育缓迟为临床表现的嘌呤核苷磷酸化酶缺陷(PNPdef)1例:经脐带血移植得以成功治疗
Objective: To explore the effect of purine nucleoside phosphorylase ( PNP) activity and exogenous hyaluronic acid ( HA) absorption after cold storage and normothermic reperfusion in rats with liver ischemia/ reperfusion injury. 目的:探讨嘌呤核苷磷酸化酶(PNP)活性和外源性透明质酸(HA)吸收率对大鼠肝脏低温保存及常温缺血再灌注损伤的作用。
The research and development of nucleoside anti-HIV drugs in recent years were reviewed. A number of pyrimidine nucleoside and purine nucleoside anti-HIV compounds were mainly introduced. Their mechanism of action and the strategy on research and development of them were also discussed. 综述近年来核苷类抗HIV药物的研究与开发,着重介绍若干嘧啶核苷类和嘌呤核苷类抗HIV化合物,并探讨其作用机制和该类药物的研发策略。
Progress on Manufacturing Purine Nucleoside with Bacillus genus 芽胞杆菌生产嘌呤核苷研究进展
6-Methylpurine-2 '-Deoxyriboside ( MePdR), an anticancer prodrug, was synthesized using MeP and dU by recombinant purine nucleoside phosphorylase of Escherichia coli. 6-甲基嘌呤-2′-脱氧核苷(MePdR)是一种新型抗癌药物,它作为药物前体应用于PNP自杀基因治疗系统可以选择性杀伤肿瘤细胞。
Enzymatic synthesis of 6-methylpurine-2 '-deoxyriboside by recombinant purine nucleoside phosphorylase Tritium labeled thymine deoxyriboside ( [ 3H] TdR) uptake was measured to observe DNA synthesis. 基因工程菌酶法合成抗癌新药6-甲基嘌呤-2′-脱氧核苷氚胸腺嘧啶核苷([3H]TdR)掺入法检测TP40对T24细胞蛋白质合成的抑制作用。
Cloning and expression of purine nucleoside phosphorylase in Escherichia coli 嘌呤核苷磷酸化酶基因的克隆及原核表达载体的构建
Study on Killing Effects of Purine Nucleoside Phosphorylase/ Fludarabine ( PNP/ fludarabine) Suicide Gene System on Human Hepatocellular Carcinoma Cell Line BEL7402 in vitro 嘌呤核苷磷酸化酶/氟达拉滨(PNP/fludarabine)自杀基因系统对人肝癌细胞系BEL7402体外杀伤效应的研究
Effect of gamma irradiation on the activity of human erythrocyte purine nucleoside phosphorylase γ线对人的红细胞嘌呤核苷磷酸化酶的影响
As a purine nucleoside, inosine is an important product in food and medicine industry. 肌苷属于嘌呤核苷,是食品和医药行业广泛应用的重要产品。
Activity of purine nucleoside phosphorylase during ischemia and reperfusion in rat liver 肝脏缺血再灌注损伤大鼠嘌呤核苷磷酸酶活性的研究
Effect of irradiation on erythrocyte purine nucleoside phosphorylase 2.some properties of the enzyme γ射线对红细胞嘌呤核苷磷酸化酶的影响二、酶的某些性质
Objective: To study the change of purine nucleoside phosphorylase ( PNP) during ischemia and reperfusion in rat liver. 目的:探讨大鼠肝脏低温保存及常温缺血再灌注过程中,不同保存液中嘌呤核苷磷酸酶(PNP)活性的变化。
Objective To construct three expression vectors harboring PNP ( purine nucleoside phosphorylase) gene under three different promoters. 目的分别构建三种启动子调控的大肠杆菌嘌呤核苷磷酸化酶(PNP)基因表达载体,检测、分析三者的表达差异。
Background: Adenosine deaminase ( ADA) is an important enzyme of purine nucleotides catabolism. It specially catalyzes the unreversible deamination of adenine nucleoside to inosine. The inosine is then oxidized to uric acid. 研究背景:腺苷脱氨酶(AdenosineDeaminase,ADA)是一种核酸分解代谢关键酶,可特异性催化腺嘌呤核苷产生不可逆脱氨反应,生成次黄嘌呤,最终氧化成尿酸排出体外。
The activity of purine nucleoside phosphorylase and release of purine were assayed by HPLC. 使用高效液相色谱技术测定嘌呤代谢产物(尿酸、黄嘌呤、次黄嘌呤、次黄苷)及嘌呤核苷磷酸化酶的活性。
We used thiourea as the sulfuration reagent of purine C-6 position, easily synthesized 6-thiopurine nucleoside analogues in ethanol under microwave irradiation. 利用硫脲作为嘌呤C-6位的硫化试剂,在乙醇中经微波辐射,简便地合成了6-硫代嘌呤核苷类似物。
Purine nucleoside analogs, as a class of important medicinal compounds, have been widely used in medical fields, such as antivirus, anticancer, anti-thrombosis and so on. 嘌呤核苷及其衍生物由于在抗病毒、抗肿瘤及抗血栓等领域具有很好的药用价值而受到人们的广泛关注,这类衍生物的合成为发现生物活性更好的药物提供依据。
Purine nucleoside compounds have a wide range of applications in the field of anti-virus, anti-tumor and anti-platelet aggregation and so on. 嘌呤核苷化合物在抗病毒、抗肿瘤以及抗血小板凝集等方面具有广泛的应用,引起了药学和化学工作者的广泛关注,成为有机化学和药物化学的重要研究领域。
Purine nucleoside phosphorylase ( PNPase, E.C.2.4.2.1) is a key enzyme in the purine salvage metabolic pathway. It could catalyze the synthesis of nucleosides, and has great potential in anti-tumour gene therapy. 嘌呤核苷磷酸化酶是嘌呤核苷补救代谢途径中的关键酶,在抗肿瘤基因治疗、核苷类药物合成等方面具有广泛的应用。